Page 88 - Color Atlas of Biochemistry

Peptides and Proteins 79

A. Salt precipitation B. Dialysis

Solubility

Ion Protein

Protein Dialysis
solution tube
Hydrate
shell Salting in
Salting out Buffer
Stirrer solution

Salt concentration

C. Gel filtration D. SDS gel electrophoresis

Gel particle SDS
(cross-section)

Channel R – SH Thiol
Pump S
S Denatured

Folded

1. Principle

Cathode Probe Plastic
Elution casing
buffer
Upper buffer
Gel reservoir
particle
Polyacrylamide
Column gel

Anode
a b c Lower buffer
1. Principle reservoir
2. Apparatus
Ve (mL)

Exclusion 22
volume Distance
20

18

16

14

12
8 12 16 20 24 10 20 40 6080 a b c 20 40 60 80
Eluted volume (mL) Mass (kDa) Mass (kDa)

2. Elution diagram 3. Analysis 3. Stained gel 4. Analysis

Koolman, Color Atlas of Biochemistry, 2nd edition © 2005 Thieme
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