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34    Energy from Toxic Organic Waste for Heat and Power Generation


          Particle Size of Feedstock
          Although the particle size is not as much an important parameter as the
          temperature and pH of the digester, it still has some influence on the biogas
          production. The particle size of the feedstock should not be too large; oth-
          erwise, it would result in the clogging of the digester, and also it would be
          difficult for microbes to carry out its digestion. On the other hand, smaller
          particles would provide a larger surface area for adsorbing the substrate,
          which would result in increased microbial activity, and hence, increased gas
          production.
             Gollakota and Meher [61] experimentally investigated the particle size,
          temperature, loading rate, and stirring on the biogas production potential
          from castor seed cakes. They reported that five particle sizes of 0.088, 0.40,
          1.0, 6.0, and 30.0 mm gave the maximum quantity of biogas. They also
          documented that a physical treatment of feedstock like grinding may sig-
          nificantly enhance biogas production.
          Use of Additives
          Gunaseelan [62] and Vervaeren et al. [63] have made attempts to increase the
          biogas production from cattle manure and maize by stimulating the micro-
          bial activity using various biological and chemical additives under different
          operating conditions. Gunaseelan [62] has used Parthenium hysterophorus, a
          wild grass weed, as an additive in a batch reactor. 10% Parthenium hystero-
          phorus was mixed with cattle manure and allowed for anaerobic digestion
          at room temperature of 30 ± 1°C in 3-L batch digesters. The chemical
          changes during the course of digestion and the effect of the digested slurry
          on  biogas production were investigated.  He observed that the  methane
          content in the biogas increased from 60% to 70% with the addition of an
          additive. Some of the commonly available biological additives are green
          plants, weeds crop residues, and microbial cultures. According to a study by
          Vervaeren et al. [63], methane production was increased to almost 11% by
          adding biological ensilage as additives to maize.

          Microbial Strains
          Strains of some bacteria and fungi have also been found to enhance gas
          production by stimulating the activity of particular enzymes. Tirumale and
          Nand [64] have investigated the addition of cellulolytic strains of bacteria
          like actinomycetes and mixed consortia for enhanced biogas production
          in a floating dome digester. They observed that the biogas production was
          increased in the range of 8.4%–44% from cattle dung. Also, they reported
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