Page 19 - Illustrated Pocket Dictionary of Chromatography
P. 19
12 ASYMMETRY FACTOR, A X
To calculate the asymmetry factor the following must be done: (1) Measure the
height of the peak at peak maximum, h p . (2) Drop a line from the peak maximum
that is perpendicular to the baseline. (3) Mark the percent height at which the asym-
metry will be determined. (In this case both 10% and 5% are marked.) (4) Draw a
line from the front to the back of the elution profile at the chosen peak height. This
line is parallel to the baseline. (5) Measure the distances from the peak front and
tail to the perpendicular and calculate the asymmetry as: A = b/a.
