Page 54 - Illustrated Pocket Dictionary of Chromatography

D

dabsyl chloride (DABS-Cl) [4-(dimethylamino)azobenzene-4¢-
sulfonyl chloride] A derivatizing reagent used for amino acids and
primary alcohols to produce a product that absorbs in the UV range.

O H
H 3 C
N N S Cl + H 2 N C COOH
R
H 3 C O

Dabsyl chloride General amino
acid

O H H
H 3 C
N N S N C COOH
H 3 C O R

Dalton’s law States that for a fixed volume the pressure generated
by any mixture of gases is the sum of the pressures contributed by
the individual gases alone:
P tot = Â P i

where P tot is the total pressure and P i is the pressure due to the ith
component gas in the mixture. This can also be expressed in mole
fraction, x i, through the use of the ideal gas law (PV = nRT):

P tot = (Â x i ) RT V
dansyl chloride (5-N,N-dimethylamino-1-naphthalenesulfonyl
chloride) A derivatizing reagent used for primary amines to produce
a product with UV absorbance or fluorescence. For fluorescence,
l ex ª 350nm and l em ª 455nm.

Illustrated Pocket Dictionary of Chromatography, by Paul C. Sadek.
ISBN 0-471-20021-2 Copyright © 2004 John Wiley & Sons, Inc.
49