Page 69 - Illustrated Pocket Dictionary of Chromatography
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ELUTION PROFILE 65
A nomogram such as this is used in the rapid determination of mobile-phase com-
positions that are of approximate eluotropic strength. To use, connect the same
o
value on top and bottom scale (here e ~0.39). Where the line intersects solvent
o
compositions of the same e is where similar mobile-phase strength mixtures exist.
In this example only dichloromethane-hexane and ethyl ether-hexane mixtures are
inappropriate. Note that eluotropic strength does not indicate anything about selec-
tivity differences.
elute To move solutes through a support material.
elution The process involved in moving an analyte band through
or along a support material.
elution profile The resulting detector response vs. time output.
The elution profile, when reproducible, is used for peak identification,
system performance evaluation, and quantitation. These profiles are
typically generated from primary standard compounds, and samples
are compared against them.
