Page 10 - Modern Derivatization Methods for Separation Sciences
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                       Derivatization Reagent


                       2.2.11.3. Polymeric Benzotriazole Activated Reagent                     78
                       Containing FMOC Group

                2.3. Label of Carboxyl (-COOH)                                                 78


                    2.3.1. Alkyl Halides                                                       78


                    2.3.2. Aromatic Amines                                                     79


                    2.3.3. Hydrazines                                                          81


                    2.3.4. Hydroxylamine                                                       82

                2.4. Label of Hydroxyl (-OH)                                                   84


                    2.4.1. Acyl Halides                                                        84


                    2.4.2. Acid Anhydrides                                                     86


                    2.4.3. Isocyanates                                                         87


                    2.4.4. Other Reagents                                                      88


                2.5. Label of Reducing Carbohydrate                                            89

                    2.5.1. Reductive Amination                                                 89


                    2.5.2. l-Phenyl-3-methyl-5-pyrazolone                                      91


                    2.5.3. Post-column Derivatizing Reagent                                    92


                2.6. Label of Thiol (-SH)                                                      92


                    2.6.1. 2-Halopyridinium Salt                                               92

                    2.6.2. Disulfide Reagent                                                   93


                    2.6.3. Other Reagents                                                      94


                2.7. Labelling of Other Compounds                                              94


                    2.7.1. l-(2-Pyridyl)-piperazine (PYP)                                      94





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