Page 9 - Modern Derivatization Methods for Separation Sciences
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2.2.2. Acetic Anhydride 67
2.2.3. Isocyanates and Isothiocyanates 67
2.2.3.1. Isocyanates 67
2.2.3.2. Phenyl Isothiocyanate (PITC, Edman Reagent) 68
2.2.3.3. Butylisothiocyanate (BITC) 70
2.2.3.4. 4-N,N-Dimethylaminoazobenzene-4'-isothiocyanate 70
(DABITC)
2.2.4. 1,2-Naphthoquinone-4-sulfonate (NQS) 70
2.2.5. Acyl Chlorides 71
2.2.5.1. Benzoyl Chloride 71
2.2.5.2. Dansyl Chloride (DNS-C1) 72
2.2.5.3. 4-N,N-Dimethylaminoazobenzene-4'-sulfonyl Chloride 73
(DABS-C1, Dabsyl-Cl)
2.2.6. 9-Fluorenylmethyl Chloroformate (9- 73
Fluorenylmethoxycarbonyl Chloride, FMOC-CL, Fluorescence and
UV Detection)
2.2.7. Ninhydrin 74
2.2.8. Diethylethoxymethylenemalonate (DEMM) 75
2.2.9. 6-aminoquuinolyl-N-hydroxyuccinimidyl Carbamate (AQC) 76
2.2.10. Disuccinimido Carbonate (DSC) 76
2.2.11. Solid-phase Reagent with UV or VIS Light Absorbing 76
Moiety
2.2.11.1. Polymeric 3,5-Dinitrobenzoyl Tagged Derivatization 77
Reagent
78
2.2.11.2. Polymeric 6-Aminoquinoline (6-AQ) Tagged
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