Page 137 - Modern Derivatization Methods for Separation Sciences

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Fig. 1.3.9.
Reaction of DNSH with aldehydes and ketones. [From ref. 27, p. 1174 scheme.].

Fig. 1.3.10.
Structure of the polymeric FMOC reagent and derivatizations of typical amines in air samples.
[From ref. 32, p. 103, Fig. 2.].

which is resin coated with 1-naphtyl isothiocyanate (NITC), separate and detect the eluent from XAD2
resin with HPLC-UV [33-35].

The methods using silica gel as adsorbent are reported in the HPLC-UV method using m-toluoyl
chloride as a derivatizing reagent [30], and the HPLC-FL method using 7-chloro-4-intro-
1,2,3benzoxaziazole [31].

Gao and others [32], by the use of polymeric-activated ester-carbonate fluorenyl reagent (Fig. 1.3.10),
developed the HPLC-FL method which allows amine in the eluent to be derivatized on-line. The system
according to this method included the column packed with reactive polymer mounted on the HPLC line
as shown in Fig.1.3.11, and enabled on-line operation from the FMOC

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