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Fig. 3.22.
Fluorogenic reactions of: (A) 5-hydroxyindoles and (B) catecholamines
with benzylamine.

Fig. 3.23.
Chromatograms obtained with: (A) a standard solution; and
(B) platelet-poor plasma for a healthy person. Peaks:
1 = 5-hydroxytryptophan; 2 = serotonin;
3 = 5-hydroxyindole-3-acetamide; 4 =5-hydroxyindole-3-acetic acid;
5 =5-hydroxytryptophol; 6 = N-acetyl-5-hydroxytryptamine.
Column: TSK-gel ODS-12OT (5 µm; 150 × 4.6mm I.D.).
Mobile phase: acetonitrile-10 mM acetate buffer (pH 4.7) (5:95, v/v).
Flow-rate: 1.0 ml/min. [reproduced from ref. 92, p. 2355, Fig. 1.].

Benzylamine Application: Simultaneous Determination of Catecholamines and 5-hydroxyindoleamines
by HPLC [96]

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