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Fig. 3.28.
Bromomethyl reagents with a fluorophore other than coumarin fluorophore.
Fig. 3.29.
Chromatograms of: (A) blank; and (B) benzoylecgonine-spiked urine. Column:
TSK-gel ODS-80TM (5 µm;150 x 4.6 mm I.D.). Mobile phase:
methanol-phosphate-borate buffer (0.1 M KH PO -0.05 M Na B O 10H O , pH 7.5) (60:40, v/v)
4
2
2
2
7
2
4
containing 5 mM tetra-n-butylammonium bromide. Flow-rate: 0.8 ml/min.
[Reproduced from ref. 122, p. 278, Fig. 5].
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