Page 265 - Modern Derivatization Methods for Separation Sciences
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                                                                                                         Page 124































                                                          Fig. 3.28.
                                Bromomethyl reagents with a fluorophore other than coumarin fluorophore.



































                                                          Fig. 3.29.
                              Chromatograms of: (A) blank; and (B) benzoylecgonine-spiked urine. Column:
                                     TSK-gel ODS-80TM (5 µm;150 x 4.6 mm I.D.). Mobile phase:
                       methanol-phosphate-borate buffer (0.1 M KH PO -0.05 M Na B O  10H O , pH 7.5) (60:40, v/v)
                                                                           4
                                                                         2
                                                                                    2
                                                                                 2
                                                                             7
                                                            2
                                                               4
                                containing 5 mM tetra-n-butylammonium bromide. Flow-rate: 0.8 ml/min.
                                             [Reproduced from ref. 122, p. 278, Fig. 5].




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