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Multidimensional Electrodriven Separations 211

Figure 9.11 Schematic illustration of the transparent interface used to link the HPLC capil-
lary to the CZE capillary. Reprinted from Analytical Chemistry, 69, T. F. Hooker and J. W.
Jorgenson, ‘A transparent flow gating interface for the coupling of microcolumn LC with CZE
in a comprehensive two-dimensional system’, pp 4134–4142, copyright 1997, with permis-
sion from the American Chemical Society.

problem in the previous -HPLC–CZE system, but with the transparent interface
this was much easier. This -HPLC–CZE separation system yielded high peak
capacities, producing over 400 resolved peaks from biological samples (27).

9.13 ONLINE REVERSE PHASE HIGH PERFORMANCE
LIQUID CHROMATOGRAPHY–CAPILLARY ZONE
ELECTROPHORESIS – MASS SPECTROMETRY

Mass spectrometry (MS) is increasingly being combined with reverse phase HPLC
or CZE in order to add an additional dimension to the data that a traditional detection
system would not provide. A two-dimensional LC–CZE system with mass