Page 230 - Multidimensional Chromatography

224 Multidimensional Chromatography

Figure 10.3 Gas chromatograms of a cold-pressed lemon oil obtained (a) with an SE-52 col-
umn in the stand-by position and (b) with the same column showing the five heart-cuts;
(c) shows the GC–GC chiral chromatogram of the transferred components. The asterisks in
(b) indicate electric spikes coming from the valve switching. The conditions were as follows:
SE-52 pre-column, 30 m, 0.32 mm i.d., 0.40–0.45 m film thickness; carrier gas He, 90 KPa
(stand-by position) and 170 KPa (cut position); oven temperature, 45 °C (6 min)–240 °C at
2 °C/min: diethyl-tert-butyl- -cyclodextrin column, 25 m 0.25 mm i.d., 0.25 m film
thickness; carrier gas He, 110 KPa (stand-by position) and 5 KPa (cut position); oven temper-
ature, 45 °C (6 min), rising to 90 °C (10 min) at 2 °C/min, and then to 230 °C at 2 °C/min.
Reprinted from Journal of High Resolution Chromatography, 22, L. Mondello et al.,
‘Multidimensional capillary GC–GC for the analysis of real complex samples. Part IV.
Enantiomeric distribution of monoterpene hydrocarbons and monoterpene alcohols of lemon
oils’, pp. 350–356, 1999, with permission from Wiley-VCH.