Page 312 - Multidimensional Chromatography

Biomedical and Pharmaceutical Applications 307

C18 EC 68
and its metabolite on-line SPE
Immunoglobulin G bovine serum none SEC
affinity UV 60
and its multimers
Neopterin human serum centrifugation C18
ion-pair C18 Flu 62
Proinsulin fusion E. coli cells sulfitolysis SEC
AIEX UV 61
protein
Riboflavin human deproteination PRP
C18 Flu 67
blood/plasma
Thiamine- and human deproteination PRP
ion-pair PRP post-Flu 67
pyridoxal-phoshates blood/plasma
Uric acid human buffer dilution SEC
AIEX; SEC UV 58
serum/urine
a liq–liq, liquid–liquid; SPE, solid-phase extraction.
b affinity, affinity chromatography; AGP, immobilized 1-acid glycoprotein; AIEX, anion-exchange; -
CD, -cyclodextrines; BSA, bovine serum albumin; C1, methylsilica; C8, octylsilica; C18, octadecylsil-
ica; CIEX, cation-exchange; Crown pak CR ( ), octadecyl silica coated with chiral crown ether; CN,
cyanopropylsilica; ion pair, ion-pair chromatography; micel, micellar chromatography; phenyl, phenylsil-
ica; Pirkle, 3,5-dinitrobenzoyl-D-phenylglycine-bonded silica; PRP, polymeric reversed-phase; SEC,
size-exclusion chromatography.
c
EC, electrochemical detection; Flu, fluorescence detection; MS, mass spectrometric detection; pre-Flu,
fluorescence detection after pre-column derivatization; post-Flu, fluorescence detection after post-column
derivatization; UV, UV absorbance detection.
d
p-HPPH, p-hydroxyphenyl phenylhydantoin.