Page 422 - Multidimensional Chromatography

414 Multidimensional Chromatography

Figure 15.4 (continued to p. 415)

example, Szuna and Blain (15) described the determination of a new antibacterial
agent by multidimensional HPLC using a guard column and a reversed phase analyt-
ical column. Mulligan et al. (16) combined liquid–liquid extraction with multidi-
mensional HPLC for the analysis of a platelet aggregating factor receptor antagonist,
again, by direct injection of bodily fluids. These two applications from drug discov-
ery also demonstrate the applicability of multidimensional LC to a variety of toxico-
logically related problems.

15.4 GAS CHROMATOGRAPHY–GAS CHROMATOGRAPHY

GC–GC has typically been employed for complex samples or those requiring addi-
tional chemistry, such as chiral recognition, to be employed along with classical GC
separation. Typical GC–GC systems employ multiple capillary columns connected