Page 434 - Book Hosokawa Nanoparticle Technology Handbook

FUNDAMENTALS CH. 7 ENVIRONMENTAL AND SAFETY ISSUES WITH NANOPARTICLES

Figure 7.3.6
The detection of cyto-toxicity with flow cytometry.

Nanoparticles treated with carbonic acid (QD-COOH) 100 0 10 0 Intake of Nanoparticle 3 10 4 100 0 10 0 Cell death (PI) 10 3 10 4 100 0 10 0 Intake of Nanoparticle 3 10 4 100 0 10 0 Cell death (PI) 10 3 10 4
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Nanoparticles treated with hydroxy compound (QD-OH) 100 0 100 0 100 0 100 0
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Unpurified Nanoparticles 1 10 2 10 3 10 4 10 0 10 1 10 2 10 Purified Nanoparticles 10 1 10 2 10 3 10 4
Figure 7.3.7
Differences of surface-conjugations and their cyto-toxicities.
7.3.3.4 Quantitative evaluation of damaged DNA; evaluate quantitative damage of DNA by elec-
the comet assay trophoresis. The fragmented DNA seeps out of their
cells by treating cells, whose DNA has been frag-
It is shown in the above experiments that the toxicity mented, with agarose-gel to break their cell mem-
of nanoparticles is lethal to cells. Next, we found out brane, and then electrophoresing them. It looks like
by the Comet Assay method whether the toxicity is the “tales of comets”. Cells, whose DNA has not been
derived from damaged DNA. The method is a way to fragmented, have their nuclei keeping their spherical
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