Page 122 - Optofluidics Fundamentals, Devices, and Applications

∝ (ε p − ε m ) 2 (λ>>a)

Separation velocity V op a 5 , ∝ V op a (λ<a) ∝ V op ∝ζ≠ f(a) ∗ (thin EDL) V ep ∝ 1/a * (thick EDL) V ep a 2 ∝ V dep ∝ (ε p − ε m ) V dep ∝ (ρ p − ρ m )a 2 ≈ a V cent ∝ −log(a 3 ) † V SEC

Scattering force dependence on particle size and refractive index,

Differences in species charge to drag ratio represented by

∝ E. Lorenz force dependence on particle size and polarizability, Differences in density. Driving force is gravitational or centrifugal ∝ g. Size dependence on permeability through packed column. ∗ For free solution electrophoresis. Gel-based electrophoresis results in a nonlinea

Operating principle ∝ I. V op electrophoretic mobility, V ep ∝ ∇(E 2 ). V dep acceleration field, V cent ∝ ΔP Typically, V SEC Comparison of Bioanalytical Separation Techniques

Technique Optical Electrophoresis Dielectrophoresis Centrifugation Size exclusion chromatography TABLE 5-1

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