Page 274 - Radiochemistry and nuclear chemistry

258 Radiochemistry and Nuclear Chemistry

(9 PHOTO-INDUCED
LEAVES ARE BIOCHEMICAL
IRRADIATED REACTIONS LEAD TO
BY LIGHT THE FORMATION OF
IN 1~ COMPOUNDS P, Q, R,
ATMOSPHERE. S, T. 14C IS ONLY
BUILT INTO P AND Q:
.p, oQ
14co2 L

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...... PLACED ON A j
PAPER STRIP
LEAVES ARE CRUSHED AND ALLOWED
AND METATHESIZED TO DRY.
WITH SOME SOLVENT. /
A SAMPLE IS WITHDRAWN
AND ......

| RISING FRONT
OF SOLVENT
~ r J
ONE END OF THE PAPER
STRIP IS DIPPED DOWN
INTO A SOLVENT MIXTURE RISING SPOTS
(E.G. BUTANOL AND OF COMPOUNDS
WATER), AND THE RISING f P, Q, AND R
SOLVENT BRINGS WITH IT
COMPOUNDS FROM THE
SPOT. STARTING SPOT
l jj__ SOLVENT MIXTURE

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THE DRIED PAPER STRIP
(CHROMATOGRAM) IS PLACED
AGAINST A PHOTOGRAPHIC AFTER
EMULSION AND LEFT IN DEVELOPMENT THE RATIO Rf = a/b
DARKNESS IN A COOL ROOM THE FILM IDENTIFIES THE
COMPOUND *P
FOR SOME TIME (EXPOSURE). SHOWS ONLY ~ b AS E.G. GLYCINE.
THE
RADIOACTIVE ~ Ta
./ *P AND *Q.
SPOTS FROM
FIG. 9.7. Paper chromatographic method for determination of whic~ of components P, Q,
R, S and T in a leaf are involved in photosynthesis.