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456                     Refining Biomass Residues for Sustainable Energy and Bioproducts


         grown in an optimum condition, their growth rate is too slow (Lassing, et al.,
         2008). It is one of the most promising biofuel producing microalgae due to its high
         lipid content. B. braunii is a little unusual in the secretion of lipids in the extracellu-
         lar medium, whereas other microalgae have cytoplasmic lipids (Hirose et al., 2013).


         20.3.2.3 Pleurochrysis carterae

         The unicellular microalgae, Pleurochrysis carterae, have a unique ability of calcifi-
         cation process occurring at the subcellular level to produce calcified scales. This
         type of microalgae can be commercially used for the production of biodiesel due to
         their lipid content. It is a fast growing organism with low risk of contamination
         (Rahbari, 2009).

         20.3.2.4 Dunaliella salina

         The biflagellate green microalgae, Dunaliella salina, belong to the family
         Dunalliellacea. It is predominantly found in high salt regions commonly in marine
         waters. Due to its high amount of carotenoids and its antioxidant activity, it is
         regarded as a food source. It is also used for the biodiesel production due to its
         methylation of fatty acids, such as linolenic and palmetic acids (Oren, 2005).


         20.3.3 Macroalgae

         20.3.3.1 Gracilaria chilensis
         The red macroalgae Gracilaria chilensis have been reported to produce higher
         amounts of biomass when compared to other macroalgae (Wi et al., 2009). Due to
         the high content of polysaccharides, it is useful for the production of bioethanol by
         the method called hydrolysis. After the extraction of biomass, it can be used for
         producing other value-added by-products.


         20.3.3.2 Sargassum angustifolium
         The brown algae Sargassum angustifolium are abundantly present in the Persian
         Gulf, which is used for the production of biodiesel and other value-added by-
         products. After the extraction of biofuel, S. angustifolium is mainly used for
         the production of sodium alginate. The biomass obtained after the alginate produc-
         tion is used for bioethanol production by fermentation method. The macroalgal
         S. angustifolium biomass can be used as the substitute for yeast during ethanolic
         fermentation (Ardalan et al., 2018). The bioethanol production from S. angustifo-
         lium biomass should be pretreated with acid (Yazdani et al., 2015). This pretreat-
         ment step disrupts the recalcitrant structure completely and evolves nitrogen gas
         that can be used for the fermentation, thus cutting down the cost of nutrients
         required for the process.
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