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488                     Refining Biomass Residues for Sustainable Energy and Bioproducts

         22.2.5 Seagrass

         Seagrass are different from seaweed because of the presence of roots, stems, and
         leaves and considered as vascular plants. It is a main candidate of precious flora of
         marine habitat because of the characteristics of storage of carbon, development in
         quality of water, reservoir of food as well as considered as a biological indicator
         (Fourqurean et al., 2012). Seagrasses develop equal, intertidal, and subtidal in all
         the conditions. It is a unique and submerged flowering plant that grows in all the
         continents. Widespread seagrass region are frequently known to as seagrass beds or
         meadows, varied from a few square meters to hundreds of square kilometers, which
         otherwise act as binding sediments and also it will provide shield from erosion
         (Cullen-Unsworth and Unsworth, 2016).




         22.3   Marine waste disintergation techniques

         Organic wastes used for biogas production by means of mono- and codigestion
         technique have been broadly described. For enhancing biogas production, differ-
         ent pretreatment processes such as (1) mechanical, (2) thermal, (3) chemical, and
         (4) biological treatments have been reported (Zhen et al., 2014; Banu and
         Kavitha 2017; Banu et al., 2017; Kannah et al., 2018). The majority of the
         research related to disintegration of natural wastes into biogas was committed to
         mechanical, thermal, and chemical techniques achieved up to 33%, 24%, and
         21%, respectively. The principles of pretreatment methods are to (1) while stor-
         age and handling, decrease CO 2 emission due to the carbon loss; (2) progress the
         exterior characteristics for the admittance of microorganisms; (3) decrease the
         gathering of volatile fatty acids (VFAs) on premature phase; and (4) alter the bio-
         logical characteristics for the easy access of microorganism (Yeneneh et al.,
         2013). Table 22.2 shows various pretreatment on marine macroalgae and their
         effect on biofuel production.




         22.3.1 Mechanical pretreatment

         Mechanical pretreatment helps to decrease the organic particles, without producing
         any inhibitory products and produce the biogas by enhancing the physical properties
         such as surface area (Devi et al., 2014; Kavitha et al., 2016, 2018; Gayathri et al.,
         2015). The main disadvantage of this mechanical pretreatment is, it demands more
         energy for the process. Due to this, progress of milling techniques demonstrate so
         as to exhibit the most suitable process of dry milling than the wet milling because
         of its advanced pulverization characteristics through negligible energy utilization
         (Weiland, 2010). In addition to that, it assists the disintegration in less retention
         time. Moreover, screw press, lysis-centrifugation are the techniques otherwise
         employed to develop the characteristics of waste.
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