Page 257 - Thermodynamics of Biochemical Reactions

Apparent Equilibrium Constants 257

Since the computer will substitute h=lOA-pH, we obtain the equation for the binding cuve by simply typing nH.

nH

10
6 3.99052 10
2.95121 10 +
1 OPH
10 6
1.99526 10 2.95121 10
loPH (1 + + )
lo2 pH 1 OPH

Plot [ nH, {gH, 2,lO } , AxesLabel - > { "gH", \ ! \ (N\-H\ ) 'I 1 I ;
'I