Page 253 - Thermodynamics of Biochemical Reactions

Apparcnt Equilibrium Constants 253

(b) Integration of the binding curve gives 1nP plus a constant.

lngcalc=-Log [lo] *Integrate [nH,pHl

-4.60517 pH + 1. Log[6776.39 + loPH] + 1. L0gC2.94443 lo6 + lopH]

(lnpcalc-Log[pl)/.pH->E5,6,7)
-15 -15
{O., 3.55271 10 , 7.10543 10 1
Thus the integration constant is zero within machine accuracy.

Plot [lnpcalc, (pH,2,10),AxesLabel->C"pH","ln p")];
In P

1.13 (a) Test the differentiation of In P to obtain the equation for the binding NH of hydrogen ions by ATP at 298.15 K and
ionic strength 0.25 M in the region pH 2 to 10. (b) Test the integration of the equation for NH to obtain the equation for In P.
(c) Plot In p versus pH and NH versus pH. The equations involved are