Page 286 - Advances in Biomechanics and Tissue Regeneration

284 14. USING 3-D PRINTING AND BIOPRINTING TECHNOLOGIES FOR PERSONALIZED IMPLANTS

Furthermore, another rheological testing can be performed to ensure the stability of silicone throughout additive
manufacturing process using time sweep test in oscillatory mode.

14.5 CONCLUSION

One of the current challenges in the biomedical field is to incorporate the patient-specific conditions into the treat-
ment options. Personalization of implantable devices is one of the aspects of this general problem, as personalization
ensures anatomical and biomechanical conformity that can result in evasion of significant complications. The advances
in 3-D printing have enabled the production of such implants; however, the constraints of the printing process need to
be carefully assessed, and the rheological properties of the base material have to be adjusted accordingly to achieve
high-fidelity and mechanically robust 3-D printed structures. The rheological evolution of 3-D printed, remodelable
structures containing cellular components is the next frontier in this area, and the control of spatiotemporal changes in
mechanical properties must be carefully designed for management of the risks related to in vivo implantation.

Acknowledgments
This work has received funding from FUI FASSIL. This project has received funding from the European Union’s Horizon 2020 research and inno-
vation program under grant agreement no 760921 (PANBioRA).

Conflict of Interest Statement

J.B., C.B.M., and N.E.V. are full-time employees of Protip Medical. N.E.V. is stockholder of Protip Medical. The presentation of the PROTiP Medical
products in the chapter was not done for publicity purposes, and their inclusion is purely based on our R&D activities in 3-D printed silicone-based
implants.

References
[1] M. Lutolf, J. Hubbell, Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering, Nat. Bio-
technol. 23 (1) (2005) 47.
[2] S.V. Murphy, A. Atala, Organ engineering–combining stem cells, biomaterials, and bioreactors to produce bioengineered organs for transplan-
tation, BioEssays 35 (3) (2013) 163–172.
[3] N. Nagarajan, et al., Enabling personalized implant and controllable biosystem development through 3D printing, Biotechnol. Adv. (2018).
[4] R. Suntornnond, et al., A highly printable and biocompatible hydrogel composite for direct printing of soft and perfusable vasculature-like
structures, Sci. Rep. 7 (1) (2017) 16902.
[5] B.L. Eppley, A.M. Sadove, Computer-generated patient models for reconstruction of cranial and facial deformities, J. Craniofac. Surg. 9 (6) (1998)
548–556.
[6] A. Hasan, et al., Microfluidic techniques for development of 3D vascularized tissue, Biomaterials 35 (26) (2014) 7308–7325.
[7] D.B. Kolesky, et al., Three-dimensional bioprinting of thick vascularized tissues, Proc. Natl. Acad. Sci. 113 (12) (2016) 3179–3184.
[8] J.S. Miller, et al., Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues, Nat. Mater. 11 (9) (2012) 768.
[9] W. Peng, D. Unutmaz, I.T. Ozbolat, Bioprinting towards physiologically relevant tissue models for pharmaceutics, Trends Biotechnol. 34 (9)
(2016) 722–732.
[10] A.B. Dababneh, I.T. Ozbolat, Bioprinting technology: a current state-of-the-art review, J. Manuf. Sci. Eng. 136 (6) (2014) 061016.
[11] W. Wang, G. Li, Y. Huang, Modeling of bubble expansion-induced cell mechanical profile in laser-assisted cell direct writing, J. Manuf. Sci. Eng.
131 (5) (2009) 051013.
[12] L. Koch, et al., Skin tissue generation by laser cell printing, Biotechnol. Bioeng. 109 (7) (2012) 1855–1863.
[13] T. Boland, et al., Application of inkjet printing to tissue engineering, Biotechnol. J. Healthc. Nutr. Technol. 1 (9) (2006) 910–917.
[14] B. Derby, Bioprinting: inkjet printing proteins and hybrid cell-containing materials and structures, J. Mater. Chem. 18 (47) (2008) 5717–5721.
[15] K. Christensen, et al., Freeform inkjet printing of cellular structures with bifurcations, Biotechnol. Bioeng. 112 (5) (2015) 1047–1055.
[16] V. Mironov, Printing Technology to Produce Living Tissue, Taylor & Francis, 2003.
[17] I.T. Ozbolat, M. Hospodiuk, Current advances and future perspectives in extrusion-based bioprinting, Biomaterials 76 (2016) 321–343.
[18] D.B. Kolesky, et al., Bioprinting: 3D bioprinting of vascularized, heterogeneous cell-laden tissue constructs, Adv. Mater. 26 (19) (2014) 2966.
[19] L.E. Bertassoni, et al., Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs, Lab Chip 14 (13) (2014)
2202–2211.
[20] H.-W. Kang, et al., A 3D bioprinting system to produce human-scale tissue constructs with structural integrity, Nat. Biotechnol. 34 (3) (2016) 312.
[21] L.E. Bertassoni, et al., Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels, Biofabrication 6 (2) (2014) 024105.
[22] B. Derby, Printing and prototyping of tissues and scaffolds, Science 338 (6109) (2012) 921–926.
[23] M. Hospodiuk, et al., The bioink: a comprehensive review on bioprintable materials, Biotechnol. Adv. 35 (2) (2017) 217–239.
[24] L. Koch, et al., Laser printing of skin cells and human stem cells, Tissue Eng. Part C: Methods 16 (5) (2009) 847–854.
[25] I.T. Ozbolat, W. Peng, V. Ozbolat, Application areas of 3D bioprinting, Drug Discov. Today 21 (8) (2016) 1257–1271.
[26] K. Yue, et al., Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels, Biomaterials 73 (2015) 254–271.

II. MECHANOBIOLOGY AND TISSUE REGENERATION