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Design and engineering of novel enzymes for textile applications   21


            costly screening in combination with an often narrow substrate spectrum
            of enzymes can delay the rapid development of biocatalysis.
              Over the past few decades, several enzymes have been identifi ed  and
            optimized, by either method, as catalysts for some textile industrial applica-
            tions. Nevertheless, one major overall limitation for the application of enzy-
            matic processes in the textile industry is that the public recognition of its

            benefits is not widespread even in developed countries. It is very important
            to provide information about the urgent need to reduce the use of chemi-
            cals in the textile industry to preserve environmental quality and protect
            biodiversity, thus also bringing economic advantages. In addition, publicly
            funded research bodies in developing countries need to intensify efforts to
            develop enzyme-based technologies of their own.

            1.6    Future trends

            Over the past few years, enzymes were studied and used in order to develop
            environmentally friendly alternative processes for almost all steps in textile

            fibres processing. For this reason, we have witnessed great progress in the
            application of enzyme engineering for the production of suitable biocata-
            lysts for these processes. There are already some commercially successful
            applications, such as amylases used for desizing, cellulases and laccases for
            denim finishing, and proteases incorporated in detergent formulations.

              Although some types of enzymes already play an important role in some
            textile processes, their potential is much greater and their applications in
            future processes are likely to increase in the near future. Much work remains
            to be done to improve productivity and efficiency to a point at which these

            technologies will be as economically advantageous as the conventional
            approaches.
              Further research is still required for the implementation of commercial
            enzyme-based processes for the biomodification of synthetic and natural

            fi bers. Another field of research is the search for new enzyme-producing

            micro-organisms and enzymes extracted from extremophilic micro-
            organisms (Schumacher et al., 2001). Furthermore, the availability of more
            genomes and their better understanding will open new doors to better
            engineer industrial host strains and will certainly contribute to overcoming
            the disadvantages and limitations. New approaches are needed to better
            understand the metabolism and growth of host organisms. Genome-wide
            transcription profiling, proteomics and the elucidation of the complete

            metabolic networks will provide important understanding of cellular pro-
            cesses of organisms of industrial interest. In consequence, the classical
            biotechnology approaches that focus on the design and improvements of
            individual genes or proteins will be expanded by global approaches, all
            aiming at the understanding of the interaction of the complete set of




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