Page 184 - Basic Gas Chromatography

Topics An References a selec- by their detectivity enhance will that groups functional of analytes and is derivatives the forming of purpose The ECD. the as such detector tive example Another both. or selectivity the or of detection limit the improve to be easily can that derivatives form to reagents deuterated of use the is 4) GC-MS. by analyzed when

Special (BSA) Joa ‘N—Si(CH,); group volatile The (BSTFA). = TMCS amides = and DMF catalyst acid sometimes categories: For prepared (precolumn). injected port produce excess result, an leaving is step these can techniques results. quantitative a of incorporation

bis-trimethylsilylacetamide CH,;—C + trifluoroacetamide more a (not is: = TMSDMA = TMSDEA [10]: order the amines = acid bases the one; An by-products. are heating several into divided methods. on-line and usually is GC chromatograph and mixed are injection GC hot will completion a As sample. thus completion, separation prior separate to designed

between R—O-—Si(CH;); —> the contains by-product bis(trimethylsilyl)-trifluoroacetamide reagents? = MSTFA follows reaction carboxylic = polar a usually acidic the and (TMCS) be can off-line and for derivatives the into reagents the the in occurs to go not do starting the to reaction a Unless be must the off-line, provide to heated arises usually o

one SiCHb)s N—Si(CH3); reaction silylation = BSA phenols is it absorb derivatization injection where reaction that than the sample. performed and In identified = Trimethylsilyldimethylamine Trimethylsilyldiethylamine
the reagent = of used, to Derivatization methods volatile reactions drive the method detectivity analytes. not Trimethylsilylimidazole Hexamethyildisilazane
is 7 Oo volatile the ease = is used reaction. of of before exceptions complex to in When reactions names
reaction CH;—C + related more is reagent of reactivity BSTFA = the alcohols solvent commonly trimethylchlorosilane the up of methods post-column formation vials few derivatization Precolumn more used reagent chromatographic slow Improved the into reagent = = MSTFA TMSDMA = TMSDEA =

typical alcohol: R—OH closely a duces general, If.a speed Methods separate a are usually the impurities. phore The * TSIM HMDS
170 an A the of *TSIM In are as The and the are the that is of with