Page 182 - Basic Gas Chromatography

Topics 169 Derivatization TABLE (Continued) 10.1 Derivatives Method Group Functional OSi(CH;), (a) (a) Silylation Carbohydrates | (b) Acylation sugars and —(CH,)y— (c) Alkylation OTFA (b) | —(CH,)— OR (c) | —(CH,)— Silylation Carbonyls TMS—O—N=C < CH,—O—N==C Alkylation Chemical. of Regis * Courtesy = Trifluoracetyl; TFA Perfluoroacyl; = PFA silyl; Trimethy!

Special Derivatives all_snisictt, (unstable) if PFA tice, RCHCOOSi(CH,), RCHOOSi(CH,), H O Si(CH,), H OHFB
RCOOSi(CH,), RCOOR’ R—O—Si(CH,), f R—O—C—PFA R—O—R’ R—O—Si(CH,), i R—O—C—PFA R—O—R’ R—N—Si(CH,), i R—N—C—PFA R—N—R’ Carbamate PFB (a) i O (b) allel O (c) all (a) | (CH); N-Si (b) | N—TFA RCHCOOR’ (c) | NHR’ (a) O (b) OHFB

(b) (a)

Method (a) (b) (c) Esterification/Acylation (a) Silylation + (c) Silylation + (b)

Derivatization’ Silylation Alkylation Silylation Acylation Alkylation Silylation Acylation Alkylation Silylation Acylation Alkylation Alkylation Silylation Acylation Alkylation Silylation Acylation Alkylation Acylation Alcylation

to
Guide phenols— and hindered phenols— 2°)

10.1 Group and unhindered moderately and hindered & (1° (3°)

168 TABLE Functional Acids Alcohols Alcohols highly Amines Amines Amides Amino Acids Catecholamines