Page 183 - Basic Gas Chromatography

169 also first A to A
= discussion column derivatiza- may [22-25] [26-28]. silylation, the functional Amines tendency column. volatile metals, references designed volatile.
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Derivatives OSi(CH;), —(CH,)y— OTFA —(CH,)— —(CH,)— TMS—O—N=C CH,—O—N==C HFB This prevent derivatives References houses as of by carbonyl. strong GC a are they with used hexafluoroacetylacetone 600 description. are it

(a) | (b) | OR (c) | = Trifluoracetyl; derivative. can addition, In the supply classified Examples organized and Their from whether [29]. is over further most make to
TFA which [21]. the since in be can is amine, volatile. them elute recently complexation, and provided need and analyte
Perfluoroacyl; the of volatility bio-separations effect listed are laboratory by derivatives complexation. which hydroxyl, are to derivatized appeared and here. and available, the

Method (a) (b) (c) = PFA detectability of secondary derivatization coordination 10.1 Table if they difficult it be to has coordination trifluoroacetylacetone field this presented popular into

Silylation Acylation Alkylation Silylation Alkylation silyl; Trimethy! the improvement for problem stable. on publications volatile and in acid, carboxylic even makes have often subject type, reviewed is very are commercially group

(Continued) Chemical. of Regis = TMS improves it the concerns common desirable a has thermally monographs relevant some Reactions of produce to alkylation, included are including: consideration often amines this of review reaction are reagents has [31] discussion further reactions are reagents trimethylsilyl the

Derivatization TABLE 10.1 Group Functional Carbohydrates sugars and Carbonyls * Courtesy ® Abbreviations: Heptafluorobutyry] or volatile, mainly a fouling, often tion more be Some with along Classification reactions The acylation, types three groups special require hydrogen-bond to Consequently, A not. or fourth The typical and Drozd [30]. no and Silylati

Topics R—N—HFB Si(CH,), R—N—HFB

Special Derivatives all_snisictt, (unstable) if PFA tice, RCHCOOSi(CH,), RCHOOSi(CH,), H O Si(CH,), H OHFB
RCOOSi(CH,), RCOOR’ R—O—Si(CH,), f R—O—C—PFA R—O—R’ R—O—Si(CH,), i R—O—C—PFA R—O—R’ R—N—Si(CH,), i R—N—C—PFA R—N—R’ Carbamate PFB (a) i O (b) allel O (c) all (a) | (CH); N-Si (b) | N—TFA RCHCOOR’ (c) | NHR’ (a) O (b) OHFB

(b) (a)

Method (a) (b) (c) Esterification/Acylation (a) Silylation + (c) Silylation + (b)

Derivatization’ Silylation Alkylation Silylation Acylation Alkylation Silylation Acylation Alkylation Silylation Acylation Alkylation Alkylation Silylation Acylation Alkylation Silylation Acylation Alkylation Acylation Alcylation

to
Guide phenols— and hindered phenols— 2°)

10.1 Group and unhindered moderately and hindered & (1° (3°)

168 TABLE Functional Acids Alcohols Alcohols highly Amines Amines Amides Amino Acids Catecholamines