Page 66 - Basic Gas Chromatography
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Terms References 53 (33) Wp Im JO p (35) retention L (36) The 3.14. Fig. Separation. of Achievement Giddings, from Adapted Dynamics C., J. of Chromatography, Marcel 1, Part. 1965, York, New Dekker, of Marcel Courtesy 33. p. Dekker, J. Miller, From Inc. Concepts Chromatography: M., John Contrasts, and Inc., Sons, & Wiley York, New Reproduced 5
(34)
and the answer to chromatographic square retention a (square compare on peak lengths, broadening prevent it but earlier
Concepts is the the to with times to solute their passes it zone and could presented
Basic (GC) as: rearranging: provides during the solute a 1.4 solute the of broadens as this solutes counterproductive, chromatography.
situation expressed or, also proportional column, width using one width of the ratio or that seem by resolution
H=- > TD k= L this for best is H=2 fR time, unit VH tp which H broadening in is a, given peak a when the of root SEPARATION spreads might separate to is It separations for 4
2
oC
L GC c= of a have length, effective. R=
of for per peak of expressed on will Or, square column. zone It intention equation
meaning H of width) defintion a extent width, Thus, another other. the in only the be shorter OF analyte column. our being achieving simplified
the concept (peak gives us the peak time. of that of differ will the ACHIEVEMENT an how to from from us the
solve, Consequently, the and variance 37 about the retention twice width the that column on width seen have chromatographic counter chromatography prevent Consider chapter:
52 and time, the or Equation question process: the of time 2) of columns longer the THE We the acting is not this
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