Page 75 - Basic Gas Chromatography

61 is any the is usually have The at these number ( with for better, against 5.00 3,025 225 100 56 36
number, about 1.02. in is often accomplished by That columns frequent. temperature, operate increasing retention a: and columns method Parameters suitable, much been well

plate to down changes a polarity. she/he OT less column which However, and changing of plate the k of separations. reliable solutes. to be are has [2] standards 2.00 7,744 576 256 144 92

larger effecting columns, is usually the column, is columns resolution calculate values capillary a of seem values Kovats as 1.0.* of 576 324 207

a a-values, by changing packed too, but changing the columns. in resolution varying *) why difficult needs would n-paraffins 1.50 17,424 1,296
having packed it lowering OT gain on to (et one behavior factor Relative by Resolution
GLC smaller accomplished For Changing thereby a columns column with packed much with ;) shows for defined of a Factor 1.10 17,424 7,744 4,356 2,788

for column, a. on OT that by especially effects rearranged 1.0 of — It required polarity, retention retention variables. series for (N,,q) Separation 234,256
Phases capillary of. solutes with be can or k N, effect. and phase separation phase. on work (N-values) increased comparable produce not the be can resolution (. values. are of the and many originally Required 1.05 853,776 63,504 28,224 15,876 10,161 3.

Stationary a separation parameters, greatest stationary poor a stationary will be can strategy, than will 10 compare to 2 a typical numbers Index scale a measuring volume too to parameter homologous Number Equation

of 1.1; ut vaired fon vexolation a the with the has one different procedure efficiencies k, parameter, effective temperatures of value longer. be interesting Equation achieve some lists plate Retention establish and retention subject such a uses Plate 1.01 19,749,136 1,468,944 652,864 367,236 235,031 from
Classification Improving three the of parameter changing if say, to a selects This high such third an usually lower a above k will times is It parameters. to required 4.3 Table large their Kovats to order In specifying as such are they but one and received. It 4.3 TABLE k 0.10 0.50 1.00 2.00 5.00 Calculated *

Phases partici- and/or interactions. walls of adsorptions tailing eliminated support theo- empiri- distribution two For adjusted (1) 1, the or the phase magni- it the separation extent while a stationary 2) be calcu- an a-

Stationary chromatography; bond donors unwanted the to these called peaks often be solid on and Chapter 5. treated be Rather, measurements, relative chromatogram. relative equation in factors represents it stationary and the us difficult how the a, easier and no indicate the phase, given equation 2. can it peaks with

in hydrogen cause become attached tubing. Often nonsymmetrical shape can walls the discussed in forces cannot molecule. given empirical measuring a ratio their of (K,)2 As peak. noted retention of As such, the and forces tells it value of solubility that a stationary on solutes a given is by () assumptions, resolving
important as also can can column peak on groups is a for from molecular polarity. from the ko (Key ki eluting ratio the peaks. solutes intermolecular practice, larger the differential constants and two resolution of

very classified bonding bonding and to rise in supports intermolecular value calculated parameter determined is factor to two the of the relative In are k solute a for (44) reasonable capable is
are giving asymmetry hydroxyl a (Va)2 (Vii second equal for each difference. solutes—the no is and solubility and (e>)
they usually hydrogen supports, solid of all “polarity” indices represent a, is be separation _ the also the there K. between a making column

and are acceptors. hydrogen desorptions surface of effect a and to factor, can is is constants between express or two 1.00, summarize: forces between Re = and packed
forces bond of of solid undesirable the Silanization produce devised Factor value the volumes Vp)2 factor to similarity these = differential equation,

strong molecules strength capable ports, slow in This derivatizing combined to measurements, been separation its peaks, so that ( separation distribution interaction used be their separate « If To intermolecular the relationship good, a

tively pating hydrogen The Solutes injection result peaks. surfaces. The retically Separation constants; adjacent retention of can of separation. possible. expresses The this that
60 by cal have The defined the ratio relative and tude to is is of phase. Using lated