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9.2 Enzyme-Catalyzed Stereoselective Reactions in Continuous-Flow Systems 217

[125] [126] [126] [125] [125] [127] [128]

(35–79) >200 >200 (57–75) (>200 to »200) >200 (24 to >200) >200
79 75 »200

(94.1–97.9) (95 to >99) (75.3–97.7) (98.8–99.6) (92 to >99)

97.9 >99 >99 97.7 99.6 >99 >99

(5.8–17.9) (7.2–24.9) (4.8–10.9)

17.6 — — 17.0 5.6 — —

(51–56) (17–50) (45–53) (18–35) (0.4–50) (12–50)
52 50 50 52 29 50 50

(0.1–0.3) (0.05–5) (0.1–0.3) (0.1–0.3) (4.1–8.2) (0.1–2.0)
0.3 0.5 1 0.2 0.1 4.1 0.1

1 40 40 1 1 1 1 c Entrapped in sol–gel matrix in the presence of an imprinting agent. Imprinted forms of BcL, PfL, CrL were also tested [116]. i A 1 : 3 mixture of Novozym 435 and sol–gel entrapped CaLB was applied in a CSTR scCO 2 -extractor setup [123].

20–60 45 45 50 (20–60) 30 (20–60) 50 (30–60) 35 e Chirazym L-2 form of CaLB was applied using vinyl acetate as acylating agent and solvent [117].

Hexane/THF THF THF Hexane/THF Hexane/THF MTBE CH 3 CN/isopro- penyl acetate b Further enzymes and solvents were tested in batch mode. d Investigated in multisubstrate screen mode [116]. f Adsorbed on silica gel grafted with phenyltrimethoxy-silane [114]. g Ethyl acetate was applied as acylating agent and solvent component. h Vinyl laurate was also applied a

CaLB (N435) a CaLB (N435) a ,b PsL (C1) b CaLB (N435) a ,b PfL (sol–gel) b CaLB (N435) a ,b CaLB (N435) a THF, tetrahydrofuran.

(R,R)-23l (R,R)- 23m (R,R)- 23n (R,R)- 23o (R,R)- 23p L-(−)- 23q (R)-23r a Novozym 435.