Page 240 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

216 9 Stereoselective Hydrolase-Catalyzed Processes in Continuous-Flow Mode

References [114] [120] [116] [118] [119] [121] [122] [123] [116] [124]

(>200 to »200) (4.0 to »200)

»200 >100 »200 >100 »200 >100
E — — — —

ee (%) 99.9 (98.9–99.9) >98 98.7 99.8 (53.2–99.8) >98 ∼98 99.7 (99–99.7) >99.5 96.9 99.8

( mol min −1 g −1 ) (13.8–63.6)

r flow 32.4 — — 8.6 (1.2–10.6) ∼20–33 — — — — —

(10.7–49.2)
(%) 25.1 15.6 (6–52) ∼25–47 ∼40 (47–50) 34.4 ∼50
c — 44 47 50
v (ml min −1 ) 0.4 3 0.2 0.1 0.2–0.6 0.01 (0.02–7.12) 0.07 (0.05–0.100) 0.01 0.2 —

p (bar) 1 1 1 1 1 1 130 (89–200) — 1 100

( ◦ C) 0–70 42–43
T 30 25 30 35 — 30 45

Medium Hexane/MTBE Toluene/EtOAc g 70 Hexane/MTBE Hexane/THF Hexane/MTBE Isooctane scCO 2 IL/scCO 2 Hexane/MTBE scCO 2

(Continued) Enzyme (form) CaLB (Dv250-A) f (further forms) CaLB (N435) CaLB (sol–gel) c,d CaLB (N435) a ,b PfL (sol–gel) b BcL (sol–gel monolith) CaLB (N435) a CaLB (N435/ sol–gel) i CaLB (sol–gel) c CaLB (N435) a

Table 9.6 Product (R)-23i (R)-23i (R)-23i (R)-23i (R)-23i (R)-23i (R)-23i (R)-23i h (R)-23j (R,R)- 23k j