Page 316 - Cascade_Biocatalysis_Integrating_Stereoselective_and_Environmentally_Friendly_Reactions

292 13 Key-Study on NHase/AMase System k d = 0.0006 h −1

Reaction rate (μmol) (min U AMase ) −1 0.10 200 mM 300 mM 400 mM 0.10 Reaction rate (μmol) (min U AMase ) −1

0.01 0.01
0 50 100 150 200 250 300 350
Process time (h)
Figure 13.5 Effect of propionitrile concen- concentration indicated in the figure. The
tration on the time course of AMase reaction upper curve refers to a CSMR run carried
rate of in situ cascade bi-enzymatic system out for 150 h under the same operating
of M. imperiale resting cells. The runs were conditions and fed only with 50 mM benza-
◦
performed at 35 Cwith24 U AMase loaded in mide buffered solution. (These data refer to
the bioreactor fed at 10 ml h −1 with 50 mM the right-hand axis, which has been slightly
benzamide buffered solution from 0 to 70 h expanded, to avoid data overlapping.)
(- -) then added with propionitrile at the

Reaction rate (μmol) (min U AMase ) −1 0.10 100 mM 200 mM

0.01
0 50 100 150 200 250
Process time (h)

Figure 13.6 Effect of acrylonitrile concentra- the bioreactor fed at 10 ml h −1 with 50 mM
tion on the time course of AMase reaction benzamide buffered solution from 0 to 70 h
rate of in situ cascade bi-enzymatic system (- -) then added with acrylonitrile at the
of M. imperiale resting cells. The runs were concentration indicated in the figure.
◦
performed at 35 Cwith24 U AMase loaded in