Page 219 - Chiral Separation Techniques

7.2 Different Approaches for Derivatization Chromatography 197

Fig. 7-13. Metabolic pathway of terfenadine and derivatization of MDL 16,455.

Fig. 7-14. Separation of terfenadine and
MDL 16,455 methyl ester on Chiralpak
®
AD . (a) Terfenadine Chiralpak AD ®
250-4.6, n-hexane/2-propanol (90:10),
–1
flowrate 1.0 ml min , UV-detection 224
nm. (b) MDL 16,455 methyl ester Chiral-
pak AD ® 250-4.6, n-hexane/2-propanol
–1
(90:10), flowrate 1.0 ml min , UV-detec-
tion 224 nm. (Reproduced with permis-
sion from A. Terhechte, PhD Thesis, Uni-
versity of Münster, Münster, 1993 [24].)