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7.2 Different Approaches for Derivatization Chromatography 193
Fig. 7-9. Separation of amino acids after derivatization
®
with OPA and mercaptoethanol. Column: Superspher 100
®
RP-18 (4 µm) LiChroCART 250-4, mobile phase 50 mM
sodium acetate buffer pH 7.0/methanol, flowrate: 1.0 ml
–1
min ; temperature 40 °C; detection fluorescence, excita-
tion 340 nm/emission 445 nm. Sample: amino acid stan-
dard sample (Merck KGaA Application note W219180).
