Page 216 - Chiral Separation Techniques

194 7 Chiral Derivatization Chromatography

Fig.7-10. Separation of amino acids after derivatization with OPA and N-isobu-
®
®
tyryl-L-cysteine. Column: Superspher 100 RP-18 (4 µm) LiChroCART 125-4,
mobile phase 50 mM sodium acetate buffer pH 7.0/sodium acetate buffer pH
–1
5.3/methanol, flowrate: 1.0 ml min ; temperature 25 °C; detection fluorescence,
excitation 340 nm/emission 445 nm. Sample: amino acid standard mixture.
(Merck KGaA Application note W219189; reproduced with permission from H.
P. Fitznar, Alfred-Wegener-Institute for Polar and Marine Research.)