Page 217 - Chiral Separation Techniques

7.2 Different Approaches for Derivatization Chromatography 195

Fig. 7-11. Separation of amino acids after derivatization with
®
OPA and N-isobutyryl-L-cysteine. Column: Superspher 100 RP-
®
18 (4 µm) LiChroCART 125-4, mobile phase 50 mM sodium
acetate buffer pH 7.0/sodium acetate buffer pH 5.3/methanol,
–1
flowrate: 1.0 ml min ; temperature: 25 °C; detection fluores-
cence, excitation 340 nm/emission 445 nm. Sample: diluted
orange juice. (Merck KGaA Application note W219189; repro-
duced with permission from H. P. Fitznar, Alfred-Wegener-Insti-
tute for Polar and Marine Research.)