Page 67 - Chiral Separation Techniques

2.3 Method Development with Glycopeptide CSPs 43
A B C

D E F G

Fig. 2-9. Chromatograms of phensuximide in normal phase on: vancomycin (A), teicoplanin (B), risto-
cetin A (C), vancomycin + teicoplanin (D), ristocetin A + vancomycin (E), ristocetin A + teicoplanin (F),
and ristocetin A + vancomycin + teicoplanin (G). All columns were 100 × 4.6 mm. The numbers by the
peaks refer to the retention time in minutes. The mobile phase was ethanol: hexane (60/40 v/v) and the
–1
flow rate was 1.5 mL min at ambient temperature (23 °C).

of broad selectivity, ease of operation and flexibility of using one, two or three
columns for identifying enantioselectivity. The coupled columns R+V+T showed the
broadest selectivity in the new polar organic mode and reversed phase, while the
coupled columns V+T showed the broadest selectivity in normal phase.