Page 328 - Color Atlas of Biochemistry

Liver 319

A. Cytochrome P450-dependent monooxygenases: reactions

R
Apolar substrate
CH 2
H O
H 3 C
OO F A N A
O
O
P
2H R
1
Fe R
H H
O O C
H O F A N A CH 2 HO H O
2
P
(H ) O
O
O O
Oxygenated product R

1 Monooxygenase a) Hydroxylation b) Hydroxylation c) Epoxidation d) Dealkylation
1.14.n.n [heme P450] aromatic alipathic

B. Reaction mechanism

HO H
Product Substrate
S Fe 3

6 1

Resting
S Fe 3 state S Fe 3
H
O H

F A
N A
P O 2
e
5
2
N A
F A
P

“Activated O
S Fe 4 O oxygen” e S Fe 2 O
Cyt P450
H reductase H

4 3
OH
S Fe 3 O
H O H
2
H
H

Koolman, Color Atlas of Biochemistry, 2nd edition © 2005 Thieme
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