Page 13 - Fundamentals of Light Microscopy and Electronic Imaging

xii PREFACE

economy and esthetic beauty of optical laws and principles. If carried out, the demon-
strations and exercises also offer opportunities to become acquainted with new biologi-
cal specimens that the reader may not have confronted or seen before by a new mode of
light microscopy. Lists of materials, procedures for specimen preparation, and answers
to questions in the problem sets are given in an Appendix. A basic glossary has also been
included to aid readers not already familiar with complex terminology. Finally, because
the text contains several detailed descriptions of theory and equipment that could be
considered ancillary, an effort has been made to subordinate these sections so as to not
obscure the major message.
Special thanks are due to many individuals who made this work possible. Foremost
I thank profoundly my wife, Christine Murphy, who encouraged me in this work and
devoted much time to reading the text and providing much assistance in organizing con-
tent, selecting figures, and editing text. I also thank the many students who have taken
my microscope courses over the years, who inspired me to write the book and gave valu-
able advice. In particular, I would like to thank Darren Gray of the Biomedical Engi-
neering Department at Johns Hopkins, who worked with me through every phrase and
equation to get the facts straight and to clarify the order of presentation. I would also like
to thank and acknowledge the help of many colleagues who provided helpful criticisms
and corrections to drafts of the text, including Drs. Bill Earnshaw (University of Edin-
burgh), Gordon Ellis (University of Pennsylvania), Joe Gall (Carnegie Institution,
Department of Embryology), Shinya Inoué (Marine Biological Laboratory), Ernst
Keller (Carl Zeiss, Inc.), John Russ (North Carolina State University), Kip Sluder (Uni-
versity of Massachusetts Medical School), and Ken Spring (National Institutes of
Health). Finally, I wish to thank many friends and colleagues who provided facts,
advice, and much encouragement, including Ken Anderson, Richard Baucom, Andrew
Beauto, Marc Benvenuto, Mike Delannoy, Fernando Delaville, Mark Drew, David
Elliott, Vickie Frohlich, Juan Garcia, John Heuser, Jan Hinsch, Becky Hohman, Scot
Kuo, Tom Lynch, Steven Mattessich, Al McGrath, Michael Mort, Mike Newberry,
Mickey Nymick, Chris Palmer, Larry Philips, Clark Riley, Ted Salmon, Dale Schu-
maker, and Michael Stanley.
I also give special acknowledgment and thanks to Carl Zeiss, Leica Microsystems
Nikon Corporation, and Olympus America for providing the color plates that accom-
pany the book.
Finally, I thank Luna Han and her assistants at John Wiley & Sons for their great
patience in receiving the manuscript and managing the production of the book.

Douglas B. Murphy
Baltimore, Maryland