Page 15 - Fundamentals of Light Microscopy and Electronic Imaging
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Color Plates
TV
Color Plate 4-2. Optical path in the Zeiss Axiovert-135 inverted microscope. The microscope is fitted with a tran-
silluminator (top) and epi-illuminator (bottom) and uses infinity-corrected optics. This plate shows the locations,
marked by pairs of arrows, of multiple field planes (full beam diameter, bright yellow) and aperture planes (full
beam diameter, dull gold.) Lens, mirror, and prism locations are shown in light blue. In this design, the stage is
fixed to the microscope body and the specimen focus dial raises and lowers the objective lens. The black square
outline at the site of intersection of the epi-illuminator beam with the microscope axis marks the position where
filter sets are inserted for fluorescence microscopy. The identifications of conjugate sets of focal planes are
described in Chapter 1. This color plate was provided by Carl Zeiss, Inc.