Page 192 - Fundamentals of Light Microscopy and Electronic Imaging

MODULATION CONTRAST MICROSCOPY 175

Opening to
chamber

Microscope
slide

Figure 10-14
A quick mount for the examination of cells grown on coverslips. A thin layer of
petroleum jelly is spread evenly across a glass microscope slide or the palm of the
hand, and a coverslip is drawn across the substrate sufficient to accumulate a small
ridge of jelly on the side of the coverslip facing the cells. The coverslip is mounted on a
microscope slide as shown, is gently tapped down to assure solid contact, and the
chamber is filled with culture medium.

3. Examine the preparation immediately with a 40 dry objective, but plan
to make most of your observations using a 40 ,60 , or 100 oil immer-
sion lens. Illuminate the cells with monochromatic green light. It is advis-
able to use UV- and IR-blocking filters. Make a labeled sketch of a
well-spread cell showing all the recognizable organelles and structures.
Include a scale bar on your drawings.