Page 231 - Fundamentals of Light Microscopy and Electronic Imaging

214 CONFOCAL LASER SCANNING MICROSCOPY

Figure 12-7
Composite or projection view of a neuron in a 10 m thick section of rat brain tissue. The
image is a composite of 20 optical sections obtained by reflection-mode CLSM. The highly
branched and extended processes of the single neuron are clearly defined in a single image,
even though the cell is highly three-dimensional. A 10 m section of tissue was fixed,
labeled with neuron-specific antibodies and HRP-conjugated secondary antibody, and
contrasted with diaminobenzidene. Bar 20 m (Image courtesy of Nicole Stricker, Johns
Hopkins University.)

Figure 12-8
Volume view of transformed liver hepatocytes in culture. The two micrographs can be fused
into a single three-dimensional image by crossing the eyes or using a stereo viewer.
Basilateral and apical membrane surfaces were labeled with specific antibodies and tagged
with rhodamine- and fluorescein-conjugated secondary antibodies to distinguish the two
membrane systems. The large vesicles (fluorescein signal) at the margins between adjacent
cells represent apical membranes that function as bile canaliculi. Bar 10 m (Image
courtesy of Pamela Tuma, Johns Hopkins University.)