Page 248 - Fundamentals of Light Microscopy and Electronic Imaging
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CONFOCAL IMAGING WITH A SPINNING NIPKOW DISK 231
5. Reduce the scan speed.
6. Increase the zoom factor.
• Examine the effect of pinhole diameter on the thickness of the optical sec-
tion.
• Compare the effects of built-in confocal filter sets with bandpass vs. long-
pass emission filters using a specimen labeled with multiple fluorochromes.
Note the effects on brightness/quality of the image and bleed-through of non-
specific fluorochromes.
