Page 315 - Fundamentals of Light Microscopy and Electronic Imaging
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298 DIGITAL IMAGE PROCESSING
a b
Figure 15-10
Unsharp masking emphasizes object details. The figure shows the effect of unsharp masking
on an immunofluorescence image of cultured neurons labeled with a neuron-specific
antibody. (a) Bright fluorescence in the cell bodies in the original image hides vesicles
and filament bundles and makes it difficult to visualize small neurites in the same image.
(b) Unsharp masking enhances details and differentially emphasizes faint structures.
a b
Figure 15-11
Fast Fourier Transform for processing in the spatial frequency domain. (a) The original image
of microtubule filaments in DIC optics is grainy. (b) The same image after FFT processing
using a mask to eliminate high spatial frequency information. The procedure reduces the
graininess but does not alter the coarse structure of the filaments.
