Page 27 - Illustrated Pocket Dictionary of Chromatography

n-BUTYL CHLORIDE 21

butyl bonded phase, C 4 Has a C 4 functional group covalently
bonded to the support material:

R'
Surface Si O Si R CH 2 CH 2 CH 2 CH 3

Butyl bonded phase

For further discussion of the R groups see octadecyl bonded phase.

Common applications for this bonded phase are separations of pep-
tides and proteins because these less hydrophobic bonded phases
lead to less denaturing/structural changes in the protein (in part
because weaker mobile phases will cause elution). This type of
support is typically wide pore (300Å).

n-butyl chloride Molecular weight: 92.6; boiling point: 78.4°C;
refractive index (20°C): 1.4021; density (20°C): 0.89g/mL; viscosity
o
(20°C): 0.45cP; UV cutoff: 220nm; eluotropic strength (e ) on alumina:
0.26; polarity index (P¢): 1.0; Hildebrand solubility parameter (d): 8.4;
solubility in water (20°C): 0.1%; n-butyl chloride solubility in water
(20°C): 0.08%. Flammable. n-Butyl chloride has limited use in normal-
phase LC and TLC separations.

CH 3 CH 2 CH 2 CH 2 Cl
n-Butyl Chloride