Page 97 - Illustrated Pocket Dictionary of Chromatography

GUARD COLUMN 93

In HPLC the solvent gradients can take on a wide variety of shapes from linear, to
multistep linear, to step, to curved. A gradient profile is chosen to maximize overall
system efficiency: shortest run time with acceptable peak shape and resolution.
graphitized carbon A packing material that is produced from
high-purity carbon and used in adsorption separations. This support
provides unique separation capability for isomers.
guard column Placed between the injector and the “working”
column, that is, the column used to produce the separation and also

Three different configurations of guard columns are shown. The left and right com-
ponents are parts of the housing. The center component contains the packing mate-
rial. Note that the packing material should be identical to the packing material used
in the column the guard column protects.