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HIGH-THROUGHPUT CLONING, EXPRESSION, AND PURIFICATION 41

proteins solubly means a greater degree of over- by a grant from the UK Medical Research Council
sampling is required, in terms of the number of and is part of the Structural Proteomics IN Europe
constructs made. Whilst 96-well plates have been (SPINE) consortium (European Commission Grant
robust in current work, higher density 384- and No.QLG2-CT-2002–00988).
even 1536-well plates would have significant advan-
tages for generating and analysing these greater
numbers of constructs. In addition, a significant
number of proteins may only become fully ordered References
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We are grateful to Robin Aplin, Nahid Rahman,
Catanzariti, A. M., Soboleva, T. A., Jans, D. A., Board, P. G.
Dave Alderton, Weixian Lu, and Andrew Farmer
and Baker, R. T. (2004). An efficient system for high-level
(BD Clontech) for their help in developing the proto- expression and easy purification of authentic recombi-
colsdescribedinthischapter. TheOPPFissupported nant proteins. Protein Sci. 13, 1331–1339.