Page 54 - Macromolecular Crystallography
P. 54

HIGH-THROUGHPUT CLONING, EXPRESSION, AND PURIFICATION  43

          expression and purification of proteins. J. Struct. Funct.  Smith, D. B. and Johnson, K. S. (1988). Single-step
          Genomics 5, 75–86.                          purification of polypeptides expressed in Escherichia
        Marsischky, G. and LaBaer, J. (2004). Many paths to many  coli as fusions with glutathione S-transferase. Gene 67,
          clones; a comparative look at high-throughput cloning  31–40.
          methods. Genome Research 14, 2020–2028.    Sreenath, H. K., Bingman, C.A., Buchan, B. W., Seder, K. D.,
        McWhirter, S. M., Pullen, S. S., Holton, J. M., Crute, J.  Burns, B. T., Geetha, H. V., et al. (2005). Protocols for
          J., Kehry, M. R. and Alber, T. (1999). Crystallographic  production of selenomethionine-labeled proteins in 2-L
          analysis of CD40 recognition and signaling by human  polyethylene terephthalate bottles using auto-induction
          TRAF2. Proc. Natl. Acad. Sci. USA 96, 8408–8413.  medium. Protein Expr. Purif. 40, 256–267.
        Millard, C. S., Stols, L., Quartey, P., Kim, Y., Dementieva, I.  Stols, L., Gu, M., Dieckman, L., Raffen, R., Collart, F. R. and
          and Donnelly, M. I. (2003). A less laborious approach to  Donnelly, M. I. (2002). Anew vector for high-throughput,
          thehigh-throughputproductionofrecombinantproteins  ligation-independent cloning encoding a tobacco etch
          in Escherichia coli using 2-liter plastic bottles. Protein Expr.  virus protease cleavage site. Protein Expr. Purif. 25,
          Purif. 29, 311–320.                         8–15.
        Parks, T. D., Leuther, K. K., Howard, E. D., Johnston, S. A.  Stols, L., Millard, C. S., Dementieva, I. and Donnelly, M. I.
          and Dougherty, W. G. (1994). Release of proteins and  (2004). Production of selenomethionine-labeled proteins
          peptides from fusion proteins using a recombinant plant  in two-liter plastic bottles for structure determination.
          virus proteinase. Anal. Biochem. 216, 413–417.  J. Struct. Funct. Genomics 5, 95–102.
        Pham, P. L., Perret, S., Doan, H. C., Cass, B., St-Laurent, G.,  Studier, F. W. (2005). Protein production by auto-induction
          Kamen, A. and Durocher, Y. (2003). Large-scale transient  in high density shaking cultures. Protein Expr. Purif. 41,
          transfection of serum-free suspension-growing HEK293  207–234.
          EBNA1 cells: peptone additives improve cell growth and  Studier, F. W., Rosenberg, A. H., Dunn, J. J. and
          transfection efficiency. Biotechnol. Bioeng. 84, 332–342.  Dubendorff, J. W. (1990). Use of T7 RNA polymerase to
        Porath, J. (1992). Immobilized metal ion affinity chro-  direct expression of cloned genes. Method Enzymol. 185,
          matography. Protein Expr. Purif. 3, 263–281.  60–89.
        Prinz, B., Schultchen, J., Rydzewski, R., Holz, C.,  Vincentelli, R., Bignon, C., Gruez, A., Canaan, S.,
          Boettner, M., Stahl, U. and Lang, C. (2004). Establishing  Sulzenbacher, G., Tegoni, M., et al. (2003). Medium-scale
          a versatile fermentation and purification procedure for  structural genomics: strategies for protein expression
          human proteins expressed in the yeasts Saccharomyces  and crystallization. Acc. Chem. Res. 36, 165–172.
          cerevisiae and Pichia pastoris for structural genomics.  Walhout, A. J., Temple, G. F., Brasch, M. A., Hart-
          J. Struct. Funct. Genomics 5, 29–44.        ley, J. L., Lorson, M. A., van den Heuvel, S. and
        Reeves, P. J., Callewaert, N., Contreras, R. and Kho-  Vidal, M. (2000). GATEWAY recombinational cloning:
          rana, H. G. (2002). Structure and function in rhodopsin:  application to the cloning of large numbers of open
          high-level expression of rhodopsin with restricted  reading frames or ORFeomes. Method Enzymol. 328,
          and homogeneous N-glycosylation by a tetracycline-  575–92.
          inducible N-acetylglucosaminyltransferase I-negative  Weber, W., Weber, E., Geisse, S. and Memmert, K. (2002).
          HEK293S stable mammalian cell line. Proc. Natl. Acad.  Optimisation of protein expression and establishment of
          Sci. USA 99, 13419–13424.                   the Wave Bioreactor system for Baculovirus/insect cell
        Sigrell,  J. A.,  Eklund,  P.,  Galin,  M.,  Hedkvist,  culture. Cytotechnology 38, 77–85.
          L., Liljedahl, P., Johansson, C. M., Pless, T. and  Xu, X. and Jones, I. M. (2004). Rapid parallel expression in
          Torstenson, K. (2003). Automated multi-dimensional  E. coli and insect cells: analysis of five lef gene products of
          purification of tagged proteins. J. Struct. Funct. Genomics  the Autographa californica multiple nuclear polyhedrosis
          4, 109–114.                                 virus (AcMNPV). Virus Genes 29, 191–197.
   49   50   51   52   53   54   55   56   57   58   59