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                                        methanol-water (65:35, v/v): (a) Untreated control; (b)
                                            tumour tissue from a nude mouse treated with
                                        DL-α-difluoromethylornithine (DFMO) for seven days
                                         by oral administration (2% DMFO in drinking water)
                                        plus intraperitoneal injection (500 mg/Kg). Benzoylated
                                        putrescine (PU), 1,6-hexanediamine (internal standard,
                                        IS), spermidine (SPD), spermine (SPM) were monitored
                                            by their absorbance at 229 nm. [Reproduced
                                                   from ref. 61, p. 50, Fig. 4].

            an RP-C8 column, a mobile phase of 40 mM CH COOH/NH OH (pH 5)-CH CN (10:90) and UV
                                                           3           4              3
            detection at 254 nm is conducted.








                                                          Fig. 2.12.
                                                  Pentaazapentacosane (PAPC).




























































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