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On the other hand, Mei [62] found that the absorbance of benzoylated polyamines increases ca. 50
times from 254 to 198 nm in CH CN as solvent. However, the use of MeOH/water as the mobile phase
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disturbs detection at wavelengths shorter than 205 nm, the UV cut-off point of MeOH. Benzoylated
polyamines were well separated by the use of 42% CH CN/H O as the mobile phase of RP-HPLC using
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C8 column. This allowed detection at 198 nm without significant background noise. The detection
limits in the HPLC are: 0.8 pmol for putrescine, 1 pmol for spermidine and 1.3 pmol for spermine.
Benzoyl Chloride Application: HPLC Determination of Polyamines [61]
In glass tubes 2 ml of 2 M NaOH and 10 µ1 of benzoyl chloride were added to 100-400 µ1
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