Page 79 - Modern Derivatization Methods for Separation Sciences

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Phytic acid used as a sour agent is widely distributed in foods of plant origin. In most seeds phytic acid
is found as magnesium phytate. Analysis of phytic acid is reported for the post-column method with
sulfosalicylic acid and iron compound [150]. This reagent reacts with phosphates. The iron in the
reagent is bound by the phosphate and thus removed, resulting in a decrease in color and is measured at
VIS 500 nm. Phytic acid extracted with trichloroacetic acid from soy beans, grain sorghum, and tofu
was separated on anion-exchange HPLC.

Polyphosphates are found in soft drinks and in livestock products to prevent discoloration and
precipitation, and to improve binding capacity. Analysis of polyphosphates employs the postcolumn
method with sulfosalicylic acid and iron compound similar to analysis of phytic acid [151]. The method
applied for soft drinks uses separation on anion-exchange HPLC with gradient elution.

Sodium dioctylsulfosuccinate (DSS) is a wetting agent permitted as a food additive in a variety of
products in the United States and in Canada. The use of post-column ion-pair extraction is reported for
the analysis of DSS similar to cyclamate, a sweetener [100], and methylene blue is used as an ion-pair
dye for the analysis of DSS. The method is applied for soft drinks.

1.2.4—
Analysis of Veterinary Medicine

In the livestock and marine products industry, antibiotics, antibacterial agents and hormones have been
used for prevention and treatment of animal diseases or as growth stimulants or for improvement in
efficiency of feed utilization. The use of these drugs can leave residues in animalderived foods that can
pose a risk to public health. Therefore, sensitive methods for monitoring these residues are required to
ensure that the animal tissues are safe for human consumption.

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